Month: February 2017

Discussion (Stress-induced reinstatement of Fix-C and Esc-C CPP)

Stress is known to precipitate relapse to drug use (Sinha, 2008) but the exact signaling molecules that govern these behavioral responses are unclear. The FST is one stressor that can be used to model stress-induced reinstatement outside the conditioning context in animal models (Kreibich & Blendy,…

Measurement of corticosterone levels

Plasma corticosterone levels were determined before and after FST. One hundred microliters whole blood was collected from mouse superficial temporal vein. The vein was punctured using a 26G needle and blood was collected in heparin coated capillary tubes. Upon completion, the wound was gently patted with…

Stress-induced reinstatement of Fix-C and Esc-C CPP

Stress-induced reinstatement was assessed using the forced swim test (FST). The FST is a common behavioral paradigm used to model stress-induced relapse to drug use (Kreibich & Blendy, 2004). Twenty-four hours after a test for CPP extinction, mice were exposed to FST. Thirty minutes prior to…

Materials and Methods (Cocaine)

Subjects Male C57BL/6J mice (8-10 weeks old) were purchased from Jackson Laboratories (Bar Harbor, Maine). Mice were housed in groups of 5/cage with food and water ad-libitum and were acclimatized to the vivarium for one week before experiments began. Animal care was in accordance with the…

Discussion about CPP

The current study employed an escalating dose schedule for cocaine-induced CPP as opposed to a typical fixed schedule used in other studies. Mice conditioned by ascending doses of cocaine maintain a higher magnitude of CPP and show greater resistance to extinction compared to mice conditioned by…

CPP Test, Extinction and reinstatement, Data Analysis

The expression of place preference was tested 3 days after the final training session in order to eliminate possible contributions from residual cocaine administration on the last training session and also to allow the consolidation of long-term memory of drug reward. Similar to habituation, CPP testing…

Materials and Methods – Animals

Male B6129S adult mice (8-10 weeks old; weighing 25-35g) were supplied from breeding colonies in our facilities at the University of Miami, Miller School of Medicine, Miami FL as previously described in detail (Balda et al., 2006). Following weaning (postnatal day 21), mice were housed in…

Discussion

Previous studies have indicated the significance of variations in the daily schedule of cocaine within the conditioning phase, rather than the dose of cocaine, on the development of drug-associated memory (Itzhak & Anderson, 2012; Conrad et al., 2013). While conditioning by fixed daily doses of cocaine…